Cell - 8 September 2016

Article

A Distinct Gene Module for Dysfunction Uncoupled

from Activation in Tumor-Infiltrating T Cells

Graphical Abstract

Highlights

d Distinct gene modules for T cell dysfunction and activation

can be uncoupled

d Single-cell profiling of CD8 TILs shows that these modules

are exclusive

d Metallothioneins, zinc regulators, promote T cell dysfunction

d CRISPR-Cas9 targeting shows Gata-3, a zinc-finger TF,

promotes dysfunction

Authors

Meromit Singer, Chao Wang, Le Cong, ...,

Vijay K. Kuchroo, Aviv Regev,

Ana C. Anderson

Correspondence

[email protected]

(V.K.K.),

[email protected] (A.R.),

[email protected] (A.C.A.)

In Brief

Single-cell profiling of tumor-infiltrating

lymphocytes identifies critical regulators

of T cell dysfunction in cancer, opening

new avenues for targeting dysfunctional

T cell states while leaving activation

programs intact.

Data Resources

GSE86042

Singer et al., 2016, Cell 166 , 1500–1511

September 8, 2016ª2016 Elsevier Inc.

http://dx.doi.org/10.1016/j.cell.2016.08.052