Cell - 8 September 2016

EF

#3 #4

cpr5-1

35S:YFP-CPR5-C / WT

Segregating T2 siblings

• transgene

• transgene

T2 sibling without transgene

T2 sibling with transgene

cpr5-1

#3 #4

cpr5-1

#3#4

cpr5-1

#3#4

cpr5-1

#3#4

-3.5

-2.5

-1.5

-0.5

0.5

1.5

2.5

-2.5

-1.5

-0.5

0.5

1.5

2.5

3.5

-1

0

1

2

3

4

PR1 PR2 PR5 Endogenous

CPR5

log

10

(relative expression to

ACT7

)

B

YFP-CPR5-C mCherry-CPR5 Merged

20 μm

N

YFP-CPR5-N YFP-CPR5

35S:YFP-CPR5-C

HA-CPR5-N HA-CPR5-C HA-CPR5 YFP-CPR5

YFP-CPR5-N

YFP-CPR5-C

α-GFP

CBB

C D

G

Dex:CPR5-C/WT vector line

(hr) 0 24 48 72 0 24 48 72cpr5

WT

cpr5-1

YFP-CPR5/cpr5-1

YFP-CPR5-C/cpr5-1

#3 #9

A

α-GFP

CBB

YFP-CPR5YFP-CPR5-C

#3 #9

PR1

EDS5

Relative expression to

UBQ5

Dex:YFP-CPR5-C/WT

H

Dex:YFP-CPR5-C

Relative expression to

UBQ5

+dex

PR1 PR2 PCR1 Chitinase AIG1

-0.5

-0.3

-0.1

0.1

0.3

0.5

Dex:YFP-CPR5-C/cpr5-1

-dex -dex +dex

(legend on next page)