Cell - 8 September 2016

0

200

400

600

800

1000

1200

1400

1600

Control

hsp-6 hsp-60 dnj-10 hsp-3 hsp-4

hsp-16.2 C12C8.1

hsp-1 F44E5.4

GFP Fluorescence (RFU)

**

**

0

10

20

30

40

50

60

70

80

90

100

hsp-4 spliced

xbp-1

hsp-70

(C12C8.1)

hsp-16.2 hsp-17 hsp-1

mRNA Fold induction after hsp-6 RNAi ER UPR Cytosolic HSR

A

B

hsp-16.2p::GFP;CF512

Control(EV)hsp-6 hsp-60 dnj-10 hsp-3 hsp-4 hsp-16.2 C12C8.1 hsp-1 F44E5.4

hsp-16.2p::GFP

Mitochondrial RNAi ER RNAi Cytosolic RNAi

Control(EV) EV:hsp-6 hsp-6:clpp-1hsp-6:dve-1hsp-6:hsf-1

Double RNAi with hsp-6

C

hsp-16.2p::GFP

200

400

600

800

1000

1200

Control

EV:

hsp-6

clpp-1:hsp-6

dve-1:hsp-6 hsf-1:hsp-6

GFP Fluorescence (RFU)^0

**

hsp-16.2p::GFP;CF512

Figure 1. Knockdown of Mitochondrial HSP70,hsp-6, Induces Cytosolic Heat Shock Response via UPRmt
(A)hsp-6andhsp-1RNAi-induced cytosolic small heat shock protein expression.hsp-16.2p::GFP induction was measured by COPAS biosorter (bottom)
(mean±SD of three biological repeats; p%0.01). EV, empty vector control RNAi.
(B) qPCR of UPRERand cytosolic HSR genes afterhsp-6RNAi (mean±SD of three biological repeats).
(C) Cytosolic HSR induced byhsp-6RNAi was dependent on bothhsf-1and UPRmtcomponents.hsp-16.2p::GFP induction was measured by COPAS biosorter
(bottom) (mean±SD of three biological repeats; p%0.001).
See alsoFigure S1and Table S1.

Cell 166 , 1539–1552, September 8, 2016 1541