161Sotiropoulou PA, Perez SA, Salagianni M et al (2006) Characterization of the optimal culture
conditions for clinical scale production of human mesenchymal stem cells. Stem Cells
24:462–471
Spiegelman BM, Green H (1981) Cyclic AMP-mediated control of lipogenic enzyme synthesis
during adipose differentiation of 3T3 cells. Cell 24:503–510
Stedman TL (2006) Stedman’s medical dictionary. Lippincott Williams & Wilkins, Baltimore, MD
Stern-Straeter J, Bonaterra GA, Juritz S et al (2014) Evaluation of the effects of different culture
media on the myogenic differentiation potential of adipose tissue- or bone marrow-derived
human mesenchymal stem cells. Int J Mol Med 33:160–170
Strem BM, Hicok KC, Zhu M et al (2005) Multipotential differentiation of adipose tissue-derived
stem cells. Keio J Med 54:132–141
Stute N, Holtz K, Bubenheim M et al (2004) Autologous serum for isolation and expansion of
human mesenchymal stem cells for clinical use. Exp Hematol 32:1212–1225
Tang QQ, Lane MD (2012) Adipogenesis: from stem cell to adipocyte. Annu Rev Biochem
81:715–736
Tang QQ, Otto TC, Lane MD (2004) Commitment of C3H10T1/2 pluripotent stem cells to the
adipocyte lineage. Proc Natl Acad Sci U S A 101(26):9607–9611
Van Der Valk J, Mellor D, Brands R et al (2004) The humane collection of fetal bovine serum and
possibilities for serum-free cell and tissue culture. Toxicol In Vitro 18(1):1–12
Van Der Valk J, Brunner D, De Smet K et al (2010) Optimization of chemically defined cell culture
media—replacing fetal bovine serum in mammalian in vitro methods. Toxicol In Vitro
24(4):1053–1063
van Vollenstee FA, Jackson C, Hoffmann D et al (2016) Human adipose derived mesenchymal
stromal cells transduced with GFP lentiviral vectors: assessment of immunophenotype and dif-
ferentiation capacity in vitro. Cytotechnology. doi:10.1007/s10616-016-9945-6
Von Heimburg D, Hemmrich K, Haydarlioglu S et al (2004) Comparison of viable cell yield from
excised versus aspirated adipose tissue. Cells Tissues Organs 178:87–92
Yang S, Pilgaard L, Chase LG et al (2012) Defined xenogeneic-free and hypoxic environment
provides superior conditions for long-term expansion of human adipose-derived stem cells.
Tissue Eng Part C Methods 18:593–602
Zimmerlin L, Donnenberg VS, Rubin JP et al (2013) Mesenchymal markers on human adipose
stem/progenitor cells. Cytometry 83(1):134–140
Zingsem J, Strasser E, Weisbach V et al (2003) Cord blood processing with an automated and
functionally closed system. Transfusion 43:806–813
Zinno F, Landi F, Scerpa MC et al (2011) Processing of hematopoietic stem cells from peripheral
blood before cryopreservation: use of a closed automated system. Transfusion 51:2656–2663
Zuk PA, Zhu M, Mizuno H et al (2001) Multilineage cells from human adipose tissue: implications
for cell-based therapies. Tissue Eng 7:211–228
Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells.
Mol Biol Cell 13:4279–4295
Zuscik MJ, Hilton MJ, Zhang X et al (2008) Regulation of chondrogenesis and chondrocyte dif-
ferentiation by stress. J Clin Invest 118:429–438
Zych J, Stimamiglio MA, Senegaglia AC et al (2013) The epigenetic modifiers 5-aza-2’-deoxycyt-
idine and trichostatin A influence adipocyte differentiation in human mesenchymal stem cells.
Braz J Med Biol Res 46:405–416
7 Isolation and Characterization of Adipose-Derived Stromal Cells