39Table 2.1
Human MSC sources , cell surface markers, and expansion media with serum supplements
SourceMethod of isolationMediaSerum supplementCell surface markersReferencesPositiveNegativeBone marrowFicoll density gradient methodDMEMFBSCD73, CD90, CD105, STRO-1CD14, CD34, CD45, HLA-DRMamidi et al. (2012),Otsuru et al. (2013) andStewart et al. (1999)Novel marrow filterdeviceDMEM-F12 ADMEMAdipose tissueDigestion methodDMEMFBSCD73, CD090, CD29, CD44, CD71, CD105, CD13, CD166, STRO-1CD14, CD31, CD34, CD45Castrechini et al. (2012),El-Kheir et al. (2014) andGronthos et al. (2001)Membrane filtrationmethodDMEM-LGFCSAmniotic fluidand membraneDensity gradient methodα-MEMFBSCD29, CD44, CD90, CD105, CD, SH2, SH3, HLA-DRCD10, CD14,CD34, HLA-DRCai et al. (2010), In ‘tAnker et al. (2003) andTsai et al. (2004)Digestion methodDMEM/F12Dental tissuesDigestion methodα-MEMFCSCD29, CD44, CD90, CD105CD14, CD34, CD45Huang et al. (2009), Kadaret al. (2009) and Seifrtovaet al. (2012)MEMFBSEndometriumDigestion methodDMEM-F12FCSCD73, CD90, CD105, CD146CD34, CD45Schuring et al. (2011)Limb budDigestion methodDMEM-LGFBSCD13, CD29,CD90, CD105, CD106CD3, CD4, CD14, CD15, CD34, CD45, HLA-DRJiao et al. (2012)Peripheral blood Ficoll density gradientα-MEMNBCSCD44, CD90, CD105, HLA-ABCCD45, CD133Ab Kadir et al. (2012)Placenta and fetal membraneDigestion methodDMEM-LGFBSCD29, CD73, CD90, CD105CD34, CD45Raynaud et al. (2012)Salivary glandDigestion method (Ringer solution)DMEMFCSCD13, CD29, CD44, CD90, STRO-1CD34, CD45Rotter et al. (2008)Skin and foreskinDigestion methodDMEM-HG DMEM DMEM-F12FBSCD44, CD73, CD90, CD105, CD166, SSEA-4, vimentinCD34, CD45, HLA-DRBartsch et al. (2005) andRiekstina et al. (2008)
(continued)2 Mesenchymal Stem Cells in Clinical Applications