Table 10.1
(continued)
Enzyme name (at gene abbreviation)
At genenumber
Lj release 3.0 genenumber
At/Lj %ID
Lj 2.5 genome clone
Genename
Reference forLjmutants
Glucanotransferase/disproportionatingenzyme (DPE2)
At2g40840
Lj1g4081830.2
71
chr1.CM1911.70.r2.m
LjDPE2
Alpha-glucan phosphorylase, plastidial(PHS1)
At3g29320
Lj2g1079510.1
72
chr2.CM1882.170.r2.a
LjPHS1
Lj0g0360239.1
69
chr6.LjB08M07.90.r2.m
Lj6g2006830.2
45*
chr6.CM0114.510.r2.m
Alpha-glucan phosphorylase, cytosolic(PHS2)
At3g46970
Lj6g2006830.2
47*
chr6.CM0114.510.r2.mchr6.LjB08M07.90.r2.m
Maltose transporter (MEX1/RCP1)
At5g17520
Lj3g3639950.2
64*
chr3.CM0127.650.r2.m
LjMEX1
Plastidic glucose translocator (pGlcT)
At5g16150
Lj0g0342169.1Lj1g3300010Lj0g0342169.2
777661
LjSGA 043891.1LjSGA 062891.1LjSGA 099348.1LjSGA 137187.1.1
LjPGlcT
List of genes coding for proteins involved in the core pathways of sucrose and starch metabolism in
Arabidopsis
and their homologues in
Lotus japonicus
. Genome clones in
L.
japonicus
were identi
fied by performing Blastp with the
Arabidopsis
protein sequences against the Miyakogusa.jp v2.5 and v3.0 (early access) genome databases developed by
the Kasuza DNA Research Institute (
http://www.kazusa.or.jp/lotus/
; Sato et al.
2008
). Unless otherwise indicated (with an asterisk), only hits with a null E-value, and a
percentage identity of sequence around 50 % were retrieved. Where the chromosome number is 0, the position of the sequence on the genome is not yet deter
mined. For
L.
japonicus
genes for which mutants and/or transgenic plants have been generated and characterised, reference is given to the corresponding publication.
Abbreviations At
Arabidopsis thaliana
;Lj Lotus japonicus
; for ADP glucose pyrophosphorylase,
LS
large subunit,
SS
small subunit. Commentary on enzymes not discussed in the text for which
there is no further information in
Lotus
: sucrose phosphate synthase and sucrose phosphate phosphatase are the two committed enzymes of sucrose synthesis in plants (Lunn and
MacRae
2003
); isoamylases 1 and 2 are involved in the synthesis of amylopectin; isoamylase 3 is involved in starch degradation (Delatte et al.
2006
; Streb et al.
2008
); limit
dextrinase catalyses a similar reaction to isoamylase, and in
Arabidopsis,
the enzyme is plastidial, but largely redundant with respect to isoamylases (Delatte et al.
2006
); glucan
water dikinase 2 is extra-plastidal and not required for starch degradation in
Arabidopsis
(Glaring et al.
2007
);
α-amylases are not required for starch degradation in
Arabidopsis
leaves (AMY3 is plastidial and may play a redundant role in starch degradation, whereas AMY1 and AMY2 are not plastidial; Yu et al.
2005
);
β-amylases 5
- 9 are not plastidial
in
Arabidopsis
; BAM7 and BAM8 are nuclear DNA-binding proteins (Fulton et al.
2008
; Reinhold et al.
2011
);
α-glucan phosphorylase 1 (PHS1) is not required for starch
degradation in
Arabidopsis
leaves in normal conditions, but may be important in stress conditions (Zeeman et al.
2004
)
108 C. Vriet et al.