Table 21.3
Mutations identi
fied in
Lotus japonicus
genes targeted by TILLING
Description
Genesymbol
Predicted genelocation
Release 3.0predicted gene
Eco-type
pre- screen
Mis-sense
Prematurestop
Splicejunction
non-coding
silent
Totalmutationsper gene
GenbankProtein ID.
Referencefor gene
ReferenceforTILLINGmutants
or Reverse
Adenylate
IPT3
chr5.CM1125.340.r2.m
Lj5g0962690.1
G
R
11
0
0
0
5
16
ABD93934.1
Isopentenyltransferaseadenylate
IPT4
chr6.CM0139.790.r2.d
Lj0g0154359.1
G
R
7
0
0
0
6
13
ABD93935.1
IsopentenyltransferaseADP glucosepyrophosphorylase LSU
APL2
chr1.CM0952.140.r2.m
Lj1g2126130.1
G
F/R
3
0
1
8
4
16
n/e
Vriet et al. 2010
ADP glucosePyrophosphorylase SSU
APS1
chr2.CM0191.60.r2.m
Lj2g3337310.1
G
R
9
0
0
5
4
18
n/e
Vriet et al. 2010
Allene oxide cyclase1
chr2.CM0903.700.r2.m
Lj2g1168900.1
G
R
11
0
0
4
1
16
n/e
Allene oxide cyclase2
chr1.CM0012.1230.r2.m
Lj1g4590880.1
G
R
3
0
0
5
5
13
n/e
Allene oxide synthase
chr5.CM0089.580.r2.d
Lj5g0080890.1
G
R
2
0
0
0
2
4
n/e
AM-induced MYBtranscription factor
MAMI
chr1.LjB18K24.70.r2.a
n/a (pseudo)
G
R
9
0
0
3
0
12
n/e
Volpe et al. 2013
Ankyrin repeat domain-containing protein
LjSGA_008026.1
Lj0g0049599.1
G
F
4
0
0
0
0
4
n/e
AP2/ERF transcription factor
chr1.CM0104.2670.r2.m
Lj1g3975310.1
G
R
12
0
0
4
4
20
n/e
AP2-EREBP transcriptionfactor
ERF1
chr5.CM0341.90.r2.m
Lj5g1167370.1
G
F/R
14
0
0
1
2
17
BAG50053.1
Asamizu et al. 2008
AP2-EREBP transcriptionfactor
ERF17
chr2.LjT11L13.40.r2.m
Lj2g0227060.1
G
F
1
0
0
0
0
1
BAG50064.1
Asamizu et al. 2008
AP2-EREBP transcriptionfactor
chr6.CM0013.730.r2.d
Lj6g1175110.1/L|6g1175120.1+
G
R
11
0
0
0
1
12
n/e
Arabinogalactan protein
chr1.CM0104.2620.r2.m
n/a
G
F
1
0
0
0
0
1
n/e
Ascorbate peroxidase
chr3.CM0616.30.r2.d
n/a
G
F
0
0
0
2
1
3
n/e
Asparaginase
NSE1
chr5.CM0096.20.r2.m
Lj5g0296030.1
G
F/R
4
0
0
5
1
10
n/e
Credali et al. 2013
Aspartyl protease family
chr1.CM0982.580.r2.d
Lj1g1584680.1
G
R
12
0
0
0
8
20
n/e
ATP Binding protein
ABCB1
chr1.CM0122.1360.r2.m
Lj1g4830300.1
G
R
14
0
0
2
0
16
n/e
Takanashi et al. 2012
ATP binding protein
chr1.LjT14I13.150.r2.a
Lj1g3137890.1
G
R
6
0
0
0
4
10
n/e
Beta-Glucosidase2
CYD2/BGD2
chr3.LjT33P07.150.r2.m
Lj0g0362549.1
G
R
0
0
0
7
1
8
ACD65510.1
Takoset al.
2010
Beta-Glucosidase4
BGD4
chr5.LjT34K16.50.r2.m
Lj5g0391410.1
G/M
R
8
0
0
9
2
19
ACD65509.2
Takoset al.
2010
bHLH DNA-binding protein
chr1.CM0147.640.r2.d
Lj0g0157999.1
G
R
6
0
0
1
2
9
n/e
Blade-on-petiole BTB/POZ-Ankyrin domain protein
chr1.CM0009.300.r2.m
Lj1g4155980.1
M
R
12
0
0
0
3
15
n/e
BOI-RELATED GENE 1,RING
finger domain protein
chr6.CM0066.230.r2.a
Lj6g1692670.1
G
R
8
0
0
1
3
12
n/e
(continued)
234 T.L. Wang and F. Robson