The Lotus japonicus Genome

Table 21.3

Mutations identi

fied in

Lotus japonicus

genes targeted by TILLING

Description

Genesymbol

Predicted genelocation

Release 3.0predicted gene

Eco-type

pre- screen

Mis-sense

Prematurestop

Splicejunction

non-coding

silent

Totalmutationsper gene

GenbankProtein ID.

Referencefor gene

ReferenceforTILLINGmutants

or Reverse

Adenylate

IPT3

chr5.CM1125.340.r2.m

Lj5g0962690.1

G

R

11

0

0

0

5

16

ABD93934.1

Isopentenyltransferaseadenylate

IPT4

chr6.CM0139.790.r2.d

Lj0g0154359.1

G

R

7

0

0

0

6

13

ABD93935.1

IsopentenyltransferaseADP glucosepyrophosphorylase LSU

APL2

chr1.CM0952.140.r2.m

Lj1g2126130.1

G

F/R

3

0

1

8

4

16

n/e

Vriet et al. 2010

ADP glucosePyrophosphorylase SSU

APS1

chr2.CM0191.60.r2.m

Lj2g3337310.1

G

R

9

0

0

5

4

18

n/e

Vriet et al. 2010

Allene oxide cyclase1

chr2.CM0903.700.r2.m

Lj2g1168900.1

G

R

11

0

0

4

1

16

n/e

Allene oxide cyclase2

chr1.CM0012.1230.r2.m

Lj1g4590880.1

G

R

3

0

0

5

5

13

n/e

Allene oxide synthase

chr5.CM0089.580.r2.d

Lj5g0080890.1

G

R

2

0

0

0

2

4

n/e

AM-induced MYBtranscription factor

MAMI

chr1.LjB18K24.70.r2.a

n/a (pseudo)

G

R

9

0

0

3

0

12

n/e

Volpe et al. 2013

Ankyrin repeat domain-containing protein

LjSGA_008026.1

Lj0g0049599.1

G

F

4

0

0

0

0

4

n/e

AP2/ERF transcription factor

chr1.CM0104.2670.r2.m

Lj1g3975310.1

G

R

12

0

0

4

4

20

n/e

AP2-EREBP transcriptionfactor

ERF1

chr5.CM0341.90.r2.m

Lj5g1167370.1

G

F/R

14

0

0

1

2

17

BAG50053.1

Asamizu et al. 2008

AP2-EREBP transcriptionfactor

ERF17

chr2.LjT11L13.40.r2.m

Lj2g0227060.1

G

F

1

0

0

0

0

1

BAG50064.1

Asamizu et al. 2008

AP2-EREBP transcriptionfactor

chr6.CM0013.730.r2.d

Lj6g1175110.1/L|6g1175120.1+

G

R

11

0

0

0

1

12

n/e

Arabinogalactan protein

chr1.CM0104.2620.r2.m

n/a

G

F

1

0

0

0

0

1

n/e

Ascorbate peroxidase

chr3.CM0616.30.r2.d

n/a

G

F

0

0

0

2

1

3

n/e

Asparaginase

NSE1

chr5.CM0096.20.r2.m

Lj5g0296030.1

G

F/R

4

0

0

5

1

10

n/e

Credali et al. 2013

Aspartyl protease family

chr1.CM0982.580.r2.d

Lj1g1584680.1

G

R

12

0

0

0

8

20

n/e

ATP Binding protein

ABCB1

chr1.CM0122.1360.r2.m

Lj1g4830300.1

G

R

14

0

0

2

0

16

n/e

Takanashi et al. 2012

ATP binding protein

chr1.LjT14I13.150.r2.a

Lj1g3137890.1

G

R

6

0

0

0

4

10

n/e

Beta-Glucosidase2

CYD2/BGD2

chr3.LjT33P07.150.r2.m

Lj0g0362549.1

G

R

0

0

0

7

1

8

ACD65510.1

Takoset al.

2010

Beta-Glucosidase4

BGD4

chr5.LjT34K16.50.r2.m

Lj5g0391410.1

G/M

R

8

0

0

9

2

19

ACD65509.2

Takoset al.

2010

bHLH DNA-binding protein

chr1.CM0147.640.r2.d

Lj0g0157999.1

G

R

6

0

0

1

2

9

n/e

Blade-on-petiole BTB/POZ-Ankyrin domain protein

chr1.CM0009.300.r2.m

Lj1g4155980.1

M

R

12

0

0

0

3

15

n/e

BOI-RELATED GENE 1,RING

finger domain protein

chr6.CM0066.230.r2.a

Lj6g1692670.1

G

R

8

0

0

1

3

12

n/e

(continued)

234 T.L. Wang and F. Robson