The Lotus japonicus Genome

Table 3.1

Selected

L. japonicus

microsatellite (simple sequence repeat,

SSR

) markers distributed over the six chromosomes (Chr.). The position is shown in centiMorgan (cM).

The quality of the markers is shown on the right side (

g

good,

vg

very good)

SSR pattern

Product size (bp)

Chr. Position Clone

Motif

Repeat numbersin MG-20

MG-20 Gifu Heteroduplex Extra bands

Separation Amplify Quality

1

0.0

TM0027 CT

33

178

158

––

vg

g

g

1

8.8

TM0050 GT

11

149

165

180

• 
  

ggg

1

31.4

TM0575 AT

23

124

110

––

ggg

1

50.1

TM0113 AAT

16

135

123

––

ggg

1

69.0

TM0295 CT

14

116

140

––

vg

g

g

2

15.6

TM0065 AT

12

114

124

130

• 
  

ggg

2

29.0

TM0377 CT

32

140

122

––

gfg

2

42.8

TM0608 AT

18

153

141

––

ggg

2

61.4

TM0522 AT

24

173

153

––

vg

g

g

2

70.6

TM0002 CT

15

159

179

––

vg

g

vg

3

10.5

TM0436 ATG

8

142

160

––

ggg

3

30.0

TM0035 AAG

17

110

95

• 
  

70

g

g

g

3

56.7

TM0049 AT

14

141

159

––

vg

g

g

3

84.8

TM0127 AAG

7 + 5

131

119

––

ggg

4

5.6

TM0256 AAAT

7

142

166

––

vg

g

g

4

19.3

TM0194 CT

17

122

156

––

vg

g

g

4

33.0

TM0030 AAT

18

142

121

• 
  

100 (weak)

vg

g

vg

4

55.4

TM0046 CT

16

155

143

––

ggg

4

69.3

TM0617 CT

13

134

122

––

ggg

5

10.4

TM0596 CT

9

106

116

––

ggg

5

29.0

TM0048 AT

12

162

146

––

ggg

5

48.6

TM0428 GT

15

143

157

161

• 
  

ggg

6

16.0

TM0302 CT

17

143

169

––

vg

g

g

6

38.5

TM0013 AT

20

206

216

• 
  

176(Gifu), 166(MG-20) g

g

g

6

48.6

TM0367 CT

27

172

152

––

vg

g

g

6

59.6

TM0336 CT

11(GT) + 14

164

180

––

ggg

24 N. Sandal and S. Sato