161will surely continue to provide us with a view of the elegant mechanisms embryos
use to solve the unique problem of transforming an egg into a multicellular
blastula.
Acknowledgments D.H. was supported by NIH grant TG 2 T32 GM007133-40 and NSF grant
1144752-IGERT, as well as the Graduate School and the College of Life Science and Agriculture
at U. Wisconsin, Madison, and thanks Danielle Grotjahn for the help and discussions with related
work. S.C. gratefully acknowledges the National Centers for Translational Research in
Reproduction and Infertility (NCTRI)/NICHD (P50 HD071836), Howard and Georgeanna Jones
Foundation for Reproductive Medicine, Medical Research Foundation of Oregon, and the Collins
Medical Trust for funding. Research in the laboratory of M.D. is supported by the National Science
Foundation (IOS-1557527). M.W. was supported by the Charles A. King Trust Postdoctoral
Fellowship Program, Bank of America, N.A., Co-Trustee. Research in the laboratory of F.P. is
supported by NIH grant RO1 GM065303.
References
Aimar C (1997) Formation of new plasma membrane during the first cleavage cycle in the egg of
Xenopus laevis: an immunocytological study. Dev Growth Differ 39:693–704
Ajduk A, Zernicka-Goetz M (2015) Polarity and cell division orientation in the cleavage embryo:
from worm to human. Mol Hum Reprod. Epub ahead of print
Amodeo AA, Jukam D, Straight AF, Skotheim JM (2015) Histone titration against the genome sets
the DNA-to-cytoplasm threshold for the Xenopus midblastula transition. Proc Natl Acad Sci U
S A 112:E1086–E1095
Azzarello A, Hoest T, Mikkelsen AL (2012) The impact of pronuclei morphology and dynamicity
on live birth outcome after time-lapse. Hum Reprod 27:2649–2657
Ballard WW (1986a) Morphogenetic movements and a provisional fate map of development in the
holostean fish, Amia calva. J Exp Zool 238:355–372
Ballard WW (1986b) Stages and rates of normal development in the holostean fish, Amia calva.
J Exp Zool 238:337–354
Basile N, Nogales Mdel C, Bronet F, Florensa M, Riqueiros M, Rodrigo L, García-Velasco J,
Meseguer M (2014) Increasing the probability of selecting chromosomally normal embryos by
time-lapse morphokinetics analysis. Fertil Steril 101:699–704
Batten BE, Albertini DF, Ducibella T (1987) Patterns of organelle distribution in mouse embryos
during preimplantation development. Am J Anat 178:204–213
Bjerkness M (1986) Physical theory of the orientation of astral mitotic spindles. Science
234:1413–1416
Black SD, Vincent J-P (1988) The first cleavage plane and the embryonic axis are determined by
separate mechanisms in Xenopus laevis. II. Experimental dissociation by lateral compression
of the egg. Dev Biol 128:65–71
Bluemink JG (1970) The first cleavage of the amphibian egg. An electron microscope study of the
onset of cytokinesis in the egg of Ambystoma mexicanum. J Ultrastruct Res 32:142–166
Bluemink JG, deLaat SW (1973) New membrane formation during cytokinesis in normal and
cytochalasin B-treated eggs of Xenopus laevis: I. Electron microscope observations. J Cell Biol
59:89–108
Boucaut JC, Darribere T, Boulekbache H, Thiery JP (1984) Prevention of gastrulation but not
neurulation by antibodies to fibronectin in amphibian embryos. Nature 307:364–367
Brachet A (1910) Experimental polyspermy as a means of analysis of fecundation. Arch
Entwiklungsmech Org 30:261–303
4 Vertebrate Embryonic Cleavage Pattern Determination