201Forristall C, Pondel M, Chen L, King ML (1995) Patterns of localization and cytoskeletal associa-
tion of two vegetally localized RNAs, Vg1 and Xcat-2. Development 121:201–208
Frey S, Richter RP, Gorlich D (2006) FG-rich repeats of nuclear pore proteins form a three-
dimensional meshwork with hydrogel-like properties. Science 314:815–817
Gagnon JA, Mowry KL (2010) Visualizing RNA localization in Xenopus oocytes. J Vis Exp
35:e1704
Gard DL (1991) Organization, nucleation, and acetylation of microtubules in Xenopus laevis
oocytes: a study by confocal immunofluorescence microscopy. Dev Biol 143:346–362
Gard DL (1992) Microtubule organization during maturation of Xenopus oocytes: assembly and
rotation of the meiotic spindles. Dev Biol 151:516–530
Gard DL (1999) Confocal microscopy and 3-D reconstruction of the cytoskeleton of Xenopus
oocytes. Microsc Res Tech 44:388–414
Gard DL, Cha BJ, King E (1997) The organization and animal-vegetal asymmetry of cytokeratin
filaments in stage VI Xenopus oocytes is dependent upon F-actin and microtubules. Dev Biol
184:95–114
Gard DL, Cha BJ, Schroeder MM (1995) Confocal immunofluorescence microscopy of microtu-
bules, microtubule-associated proteins, and microtubule-organizing centers during amphibian
oogenesis and early development. Curr Top Dev Biol 31:383–431
Ge X, Grotjahn D, Welch E, Lyman-Gingerich J, Holguin C, Dimitrova E, Abrams EW, Gupta T,
Marlow FL, Yabe T et al (2014) Hecate/Grip2a acts to reorganize the cytoskeleton in the
symmetry- breaking event of embryonic axis induction. PLoS Genet 10:e1004422
Ghosh S, Marchand V, Gaspar I, Ephrussi A (2012) Control of RNP motility and localization by a
splicing-dependent structure in oskar mRNA. Nat Struct Mol Biol 19:441–449
Glotzer JB, Saffrich R, Glotzer M, Ephrussi A (1997) Cytoplasmic flows localize injected oskar
RNA in Drosophila oocytes. Curr Biol 7:326–337
Gore AV, Maegawa S, Cheong A, Gilligan PC, Weinberg ES, Sampath K (2005) The zebrafish
dorsal axis is apparent at the four-cell stage. Nature 438:1030–1035
Grey RD, Wolf DP, Hedrick JL (1974) Formation and structure of fertilization envelope in Xenopus
laevis. Dev Biol 36:44–61
Gupta T, Marlow FL, Ferriola D, Mackiewicz K, Dapprich J, Monos D, Mullins MC (2010)
Microtubule actin crosslinking factor 1 regulates the Balbiani body and animal-vegetal polarity
of the zebrafish oocyte. PLoS Genet 6:e1001073
Han TW, Kato M, Xie S, Wu LC, Mirzaei H, Pei J, Chen M, Xie Y, Allen J, Xiao G et al (2012)
Cell-free formation of RNA granules: bound RNAs identify features and components of cel-
lular assemblies. Cell 149:768–779
Hart NH, Becker KA, Wolenski JS (1992) The sperm entry site during fertilization of the zebrafish
egg: localization of actin. Mol Reprod Dev 32:217–228
Hashimoto Y, Maegawa S, Nagai T, Yamaha E, Suzuki H, Yasuda K, Inoue K (2004) Localized
maternal factors are required for zebrafish germ cell formation. Dev Biol 268:152–161
Havin L, Git A, Elisha Z, Oberman F, Yaniv K, Schwartz SP, Standart N, Yisraeli JK (1998) RNA-
binding protein conserved in both microtubule- and microfilament-based RNA localization.
Genes Dev 12:1593–1598
Heasman J (2006) Maternal determinants of embryonic cell fate. Semin Cell Dev Biol 17:93–98
Heasman J, Quarmby J, Wylie CC (1984) The mitochondrial cloud of Xenopus oocytes: the source
of germinal granule material. Dev Biol 105:458–469
Heim AE, Hartung O, Rothhamel S, Ferreira E, Jenny A, Marlow FL (2014) Oocyte polarity
requires a Bucky ball-dependent feedback amplification loop. Development 141:842–854
Heinrich B, Deshler JO (2009) RNA localization to the Balbiani body in Xenopus oocytes is regu-
lated by the energy state of the cell and is facilitated by kinesin II. RNA 15:524–536
Hertig AT (1968) The primary human oocyte: some observations on the fine structure of Balbiani’s
vitelline body and the origin of the annulate lamellae. Am J Anat 122:107–137
Hiiragi T, Solter D (2004) First cleavage plane of the mouse egg is not predetermined but defined
by the topology of the two apposing pronuclei. Nature 430:360–364
5 Localization in Oogenesis of Maternal Regulators of Embryonic Development