549germ cell (GC) development
cytoplasm rearrangements, 389
genes, 387–388
oogenesis, 386, 389
pPGC, 386
germ plasm formation
Bb, 389, 391
buckyball, 391
large-scale mutagenesis, 391
germ plasm localization, 390
PGCs, 391
migration, 393–395
post-transcriptional regulation,
392, 393
somatic gonadal environment, 386
Zygote, 100
Zygotic, 489
Dicer mutant fish, 497
microRNAs, 500
miR-430 miRNA, 497
miRNAs, 497, 498, 508
mRNA expression, 507
nucleus, 491
rRNA expression, 509
transcription, 494, 503
transcripts, 490
ZGA (see Zygotic genome activation
(ZGA))
Zygotic genome activation (ZGA), 101, 104,
142, 507–508
Zygotic transcription, early embryo, 458–472,
474, 475
detection
identification, pre-MBT mRNAs,
459–461
limitations, 465–466
mammals, 465
metabolic labeling, 459
pre-MBT embryos, 458
Xenopus, 462–463
zebrafish, 463–465
gene activation and MBT, vertebrate
embryos, 444
gene expression, 466–467
histone modifications
activation and repression, 474
DNA methylation, 475
H3K4me3, H3K27me3, and RNAPII, 474
regulation, 475
Surveys, 474
zebrafish, 475
large scale genome activation
diverse model organisms, 468
mechanisms, repression and N,
468–472
C ratio (see Nuclear cytoplasmic ratio,
zygotic transcription)
wave of transcription, 468
Xenopus and zebrafish, 467
miR-427/430, 462
oocyte maturation, 456–457
repression, pre-MBT embryos, 457–458
zebrafish, 460
zygotic gene activation, 457Index