© Springer International Publishing AG 2017 49
T. Angelone et al. (eds.), Chromogranins: from Cell Biology to Physiology
and Biomedicine, UNIPA Springer Series, DOI 10.1007/978-3-319-58338-9_4
Involvement of Chromogranin A and Its Derived
Peptides to Fight Infections
Marie-Hélène Metz-Boutigue and Francis Schneider
Abstract In this chapter, after the presentation of the expression of Chromogranin
A and its antimicrobial derived peptides in circulation, we report a clinical analysis
for Vasostatin-I (VS-I) and the preparation of antimicrobial coating with Cateslytin
(CTL). In a clinical multicenter analysis of VS-I in the plasma of critically ill
patients (481 patients and 13 healthy staff), we have shown for the first time, that an
increase in the VS-I concentration was present on admission in non-selected criti-
cally ill patients and that it was associated with poor outcome at day 28 after admis-
sion. By using a combination of VS-I, lactate and age values, our assessment of
prognosis was better than taking the parameters alone. Concerning the preparation
of antimicrobial biomaterial we decided to use polysaccharide multilayer film based
on CTL functionalized hyaluronic acid as polyanion and chitosan as polycation,
(HA-CTL-C/CHI), with the aim of designing a self-defensive coating against both
bacteria (Staphylococcus aureus) and yeast (Candida albicans). The ability of S.
aureus and Candida species to degrade HA, by producing hyaluronidase, allows the
active peptide to be released from film only in the presence of the pathogens. We
have demonstrated the antimicrobial activities of HA-CTL in solution and
HA-CTL-C/CHI films against S. aureus and C. albicans. We have shown the pene-
tration of the fluorescently labeled HA FITC -CTL-C into the cell membrane of C.
albicans and the cytotoxicity of HA-CTL-C/CHI films was tested through human
gingival fibroblasts (HGFs) viability.
M.-H. Metz-Boutigue (*)
Inserm U1121, Université de Strasbourg, FMTS, Faculté de Chirurgie Dentaire Hôpital Civil,
Porte de l’hôpital, 67000 Strasbourg, France
e-mail: [email protected]
F. Schneider
Inserm U1121, Université de Strasbourg, FMTS, Service de Réanimation Médicale, Hôpital
Hautepierre, Avenue Molière, 67000 Strasbourg, France
e-mail: [email protected]