4.3.5 Monitoring of Cell Activity Change to External
Stimulations
We demonstrated the electrical recording capabilities of nanoES in a 3D
cardiomyocyte/nanoES construct (Fig.4.16a). Output recorded from a single
nanowire FET inside construct showed regular spikes with1 Hz frequency, 2
to 3 mV calibrated amplitude,3 signal-to-noise (S/N) ratio, and*2 ms width.
Fig. 4.15 Biocompatibility of nanoES. 50-μm projection confocalfluorescence images of neuron
culture in Matrigel™(a) versus nanoES/Matrigel™(b). Neurons were labeled by LIVE/DEAD
cytotoxicity assay after 0 days (I), 7 days (II), 14 days (III) and 21 days (IV) culture.
cPercentage of viable hippocampal neurons cultured in nanoES/Matrigel™versus Matrigel™. Cell
viability was evaluated with LIVE/DEAD cytotoxicity assay from (a,b). Cells were counted from
3D reconstructed confocal fluorescence photomicrographs. n = 6; data are means±SD.
Differences between groups were very small although statistically significant (p< 0.05).dMTS
cytotoxicity assay of cardiomyocytes evaluated using the MTS assay. n = 6; data are
means±SD. Differences between groups were very small although statistically significant
(p< 0.05)
56 4 Three-Dimensional Macroporous Nanoelectronics...