gold coverslips were used again to protect from light and allowed for 12 h of
clearance before imaging. When the antibody solutions werefirst prepared, they
included 0.3 Triton X-100 and 5% normal goat serum.
Chronic immunostaining for lateral ventricle: Slices were cleared with 5 mg/
mL sodium borohydride in HBHS for 30 min, with 3 following washes with HBHS
in 5–10 min intervals. Then, slices were incubated with 0.5% (v/v) Triton X-100 in
HBHS for 30 min at room temperature. Next, sections were blocked with 5% (w/v)
serum and incubated overnight at room temperature. Next, slices were washed four
times in 30-minute intervals with HBHS to clear any remaining serum in the tissue.
Slices were then incubated with primary antibody NeuN in 0.3% Triton-X100 and
3% serum in PBS overnight at room temperature. After 24 h, sections were washed
four times for 30 min in PBS and then counterstained with Hoechst 33342. Prolong
gold coverslips were used again to protect from light and allowed for 12 h of
clearance before imaging. When the antibody solutions werefirst prepared, they
included 0.3 Triton X-100 and 5% serum.
5.3 Results and Discussion................................
5.3.1 Syringe-Injectable Electronics......................
Figure5.6shows the schematics of the basic idea. We design and encapsulate
electronic components in a mesh network made by photopatternable epoxy (SU-8).
This mesh network are fabricated on a nickel sacrificial layer as reported previously
[ 14 ], and then completely removed from the substrate. All the electronic sensor
Fig. 5.6 Syringe-injectable electronics.aMesh electronics is loaded into syringe and injected into
targeted system.bMesh electronics is injected into targeted system and gradually unfold.cMesh
electronics has been injected into targeted system and totally unfold with I/O region injected
outside for bonding. Blue and green dots: nanodevice and (Continued) I/O pads; yellow: metal line
encapsulated in polymer; red: supporting polymer; blue: solution
78 5 Syringe Injectable Electronics