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hand, the excellent resistance to FHB observed in 7E(7A), 7E(7B), and 7E(7D)
substitution lines (Fig. 11.1 ), was shown to be due to the presence of a gene(s) for
type II resistance on the 7EL arm (Shen et al. 2004 ; Shen and Ohm 2006 ). Transfer
of this effective gene in both bread and durum wheat has been recently undertaken
(Forte et al. 2011 and unpublished).
Molecular and cytogenetic techniques were used to identify a new wheat- Th.
intermedium ssp. trichophorum substitution line from the cross of TE-3 partial
amphiploid and the Chinese wheat line ML-13 (Hu et al. 2011 ). The Thinopyrum
derived St-chromosome, substituting for wheat chromosome 1D, was proved to
contain new gene(s) for stripe rust resistance. Furthermore, wheat- Th. intermedium
lines carrying a group 4 homoeologous chromosome or its short arm were resistant
to eyespot (Li et al. 2005 ). Eyespot resistance was similarly associated to a group 4
chromosome of Th. ponticum , but the same was not true for group 4 chromosomes
of the diploid Th. elongatum and Th. bessarabicum species (Li et al. 2005 ).
On the other hand, taking into account tolerance to abiotic stresses, chromo-
somes of diploid Thinopyrum species with different homoeology to wheat chromosomes
Fig. 11.1 Partial somatic metaphase plate of a bread wheat- Thinopyrum elongatum 7E(7D) sub-
stitution line, subjected to fl uorescence GISH, as described in Forte et al. ( 2014 ). Total Th. elonga-
tum DNA, used as one probe, is marked by green fl uorescence (FITC fl uorochrome), which
highlights the two 7E chromosomes ( arrowed ). Total wheat DNA, used as the other probe, was
labelled by Cy3 (red fl uorescence). The more greenish wheat chromosomes belong to the D
genome, with which the Thinopyrum DNA cross hybridizes more intensely (i.e., shares higher
homology) than with those of the A and B genomes
C. Ceoloni et al.