Multiphase Bioreactor Design

Table 14.2 Other examples of (non-commercial)

large-scale (>500 L) systems

System Company Scale/Operating Details Reference

Aralia cordata PCC Technology Inc.,
Japan

500 L STR

N: 30 rpm

semi-continuous

Kobayashi et al.

(1993)

Catharanthus roseus Metabogal, Israel 3000 L STR
semi-continuous

Shaaltiel (1999)

Catharanthus roseus GBF, Germany 5000 L STR
D: 0.5 m; N: 30 rpm
3 Rushton turbines batch

Schiel & Berlin

(1987)

Echinacea purpurea
Panax ginseng
Rauwolfia
serpentina

Diversa, Germany 75, 750, 7500, 15 000, 75 000

L

2-stage Intermig impellers;

batch/semi-continuous mode

Ritterhaus et al.

(1990)

Lithospermum
erythrorhizon

Mitsui Chemicals Inc.,

Japan

1000 L RDR Takahashi & Fujita

(1991)

Nicotiana tabacwn Japan Tobacco Inc.,
Japan

20 000 L STR (see Table 4)

batch, continuous

Hashimoto &

Azechi (1988)

1500 L ALR Kato et al. (1976)

Panax ginseng Omutinsk Chemical
Plant, Russia

630 L STR

D: 0.9 m; N: 50 rpm

Shamakov et al.

(1991)

Taxus spp. ESCAgenetics
Corporation, USA

2500 L STR Smith (1995)

Table 14.3 Comparison of biocatalyst properties

Biocatalyst Shape Size
(μm)

Cell

wall

Aggregates Doubling

time (h)

Shear

tolerance

OUR

(mmol

L−^1 h−^1 )

Plant Cells Spherical-
Cylindrical

1:100–

500

d: 20–

50

Yes Yes 20–100 low-

moderate

101

Animal
Cells

Spherical d: 10–

20

No No 20 low 10 –1

Bacteria Spherical
Cylindrical
Spiral

d: <l

1: <5

Yes Yes/No 0.5–10 high 103

Yeasts Spherical d: 5–10 Yes Yes/No 10 high 102

Moulds Mycelial d: 5–10 Yes Yes/No 10–20 moderate-10^2

Multiphase bioreactor design 420