Figure 15.4 Rise-dependent killing
volume as a function of: A) bubble
diameter and B) reactor height.
Required contact times for attachment
are 0, 0.1 and 1 second. Markers
shown are points where the killing
volume is calculated.
additives and with additives as calculated from the model of Meier et al. (1999). Without
any protective compounds the contact time is 0.01 s. For a 3.5 mm bubble and a 15 μm
cell the projected radius is 9.2 μm, which means that for each centimetre increase in
height the Vd, rise increases with 2.6 10−^12 m^3. Assuming a killing volume of 8.6 10−^10 m^3
Lethal effects of bubbles in animal-cell culture 471