Table 13.1
In vitro protocols developed for
Cannabis sativa
L.
Explant
Response
Medium
Reference
Seedling parts
Cell suspension cultures
Modi
fied Gamborg
’s medium
Veliky and Genest (
1972
)
Root, hypocotyl, leaves of seedling, male andfemale
floral parts
Callus cultures
MS + 0.1
- 0.01 ppm
KIN + 1.0 ppm 2,4-D
Itokawa et al. (
1975
)
Seedling parts
Cell suspension cultures
Itokawa et al. (
1977
)
Embryo, leaf, stem
Callus and cell suspension cultures
MS + 3 mg/l 2,4,5-T
Loh et al. (
1983
)
Seedling parts
Cell suspension cultures
MS + B5 vitamins + 3 mg/l2,4,5-T
Hartsel et al. (
1983
)
Leaf
Cell suspension cultures
B5 + 0.5 mg/l KIN + 1 mg/l 2,4 -D
Braemer et al. (
1987
)
Anthers
Cell suspension cultures: cryopreservation
10% DMSO
Jekkel et al. (
1989
)
Leaf
Cell culture
MS + B5 vitamins + 1 mg/l2,4-D + 1 mg/l KIN
Flores-Sanchez et al.(^2009
)
Leaf
Callus culture
MS + 0.5
μM NAA + 1.0
μM
TDZ
Lata et al. (
2010
)
Internodes
Callus culture
MS + 1 mg/l BAP + 0.5 mg/lNAA
Jiang et al. (
2015
)
Cotyledon
Callus culture
MS + 2 mg/l TDZ + 0.5 mg/LIBA
Movahedi et al. (
2015
)
Stem and leaf segment from seedling
Callus culture; Agrobacterium mediatedtransformation
MS + B5 vitamins + 5
μM 2,4
D+1
μM KIN
Feeney and Punja (
2003
)
Hypocotyl
Hairy root cultures
A. rhizogenes and A. tumefaciensstrains
Wahby et al. (
2006
,^2013
)
Seedling
Hairy root cultures from callus
B5 + 4 mg/l NAA
Farag and Kayser (
2015
)
Root development from callus
Hemphill et al. (
1978
)
Root development from callus
Fisse et al. (
1981
)
Stem, cotyledon, root
Callus formation
MS + NAA
Fisse and Andres (
1985
)
(continued)
13 Micropropagation ofCannabis sativaL.—An Update 289