Oono Y, Ooura C, Rahman A, Aspuria ET, Hayashi KI, Tanaka A, Uchimiya H (2003)
p-Chlorophenoxyisobutyric acid impairs auxin response in Arabidopsis root. Plant Physiol
133:1135– 1147
Pacifico D, Miselli F, Micheler M, Carboni A, Ranalli P, Mandolino G (2006) Genetics and
marker-assisted selection of the chemotype inCannabis sativaL. Mol Breed 17:257– 268
Palazón J, CusidóRM, Roig C, Pinñol MT (1997) Effect ofrolgenes fromAgrobacterium
rhizogenesTL-DNA on nicotine production in tobacco root cultures. Plant Physiol Biochem
35:155– 162
Pistelli L, Giovannini A, Ruffoni B, Bertoli A, Pistelli L (2010) Hairy root cultures for secondary
metabolites production. In: MT Giardi, G Rea, B Berra (eds) Bio-farms for nutraceuticals:
functional food and safety control by biosensors, landes. Bioscience and Springer Science
+Business Media, pp 167– 184
Ranalli P, Di Candilo M, Mandolino G, Grassi G, Carboni A (1999) Hemp for sustainable
agricultural systems. Agro-Food-Ind Hi-Tech 2:33– 38
Shkryl YN, Veremeichik GN, Bulgakov VP, Zhuravlev YN (2011) Induction of anthraquinone
biosynthesis inRubia cordifoliacells by heterologous expression of a calcium-dependent
protein kinase gene. Biotechnol Bioeng 108:1734– 1738
Sirikantaramas S, Morimoto S, Shoyama Y, Ishikawa Y, Wada Y, Shoyama Y (2004) The gene
controlling marijuana psychoactivity: molecular cloning and heterologous expression of
D1-tetrahydrocannabinolic acid synthase fromCannabis sativaL. J Biol Chem 279:39767–
39774
Stark MM, Englehart K, Sexton BF, Tubridge R, Jackson P (2003) Use of a pupillometer to assess
change in papillary size post-cannabis. J Clin Forensic Med 10:9– 11
Stiller J, Martirani L, Tuppale S, Chian RJ, Chiurazzi M, Gresshoff PM (1997) High frequency
transformation and regeneration of transgenic plants in the model legumeLottus japonicus.
J Exp Bot 48:1357– 1365
Taura F (2009) Studies on tetrahydrocannabinolic acid synthase that produces the acidic precursor
of tetrahydrocannabinol, the pharmacologically active cannabinoid in marijuana. Drug Discov
Ther 3:83– 87
Tarkowski P, Vereecke D (2014) Threats and opportunities of plant pathogenic bacteria.
Biotechnol Adv 32:215– 229
Tiwari RK, Trivedi M, Guang ZC, Guo GQ, Zheng GC (2007) Genetic transformation
ofGentiana macrophyllawithAgrobacterium rhizogenes: growth and production of secoiri-
doid glucoside gentiopicroside in transformed hairy root cultures. Plant Cell Rep 26:199– 210
Trémouillaux-Guiller J (2013) Hairy Root Culture: An Alternative Terpenoid Expression Platform.
In: Ramawat KG, Mérillon JM (eds) Natural Products. Springer, Berlin-Heidelberg, pp 2941–
2970
Turner CE, Elsohly MA, Boeren E (1980) Constituents ofCannabis sativaL. XVII. A review of
the natural constituents. J Nat Prod 43:169– 234
Veliky IA, Genest K (1972) Growth and metabolites ofCannabis sativacell suspension cultures.
Lloydia 35:450– 456
Wahby I (2007) Aproximaciones biotecnológicas tendentes a la mejora del cáñamo (Cannabis
sativaL.): obtención y cultivo de raíces transformadas, trasformación genética y regeneración
in vitro. PhD Dissertation, University of Granada
Wahby I, Caba JM, Ligero F (2002) Susceptibilitédu chanvre (Cannabis sativaL.)àl’infection
parAgrobacterium. Congress Les Biotechnologies: Quelles Opportunités pour le Maroc?,
Rabat, Morocco, p 71
Wahby I, Caba JM, Recalde L, Ligero F (2004) Assessment ofAgrobacterium-mediated genetic
transformation in hemp (Cannabis sativaL.). Congress International de Biochimie, Marrakech,
Morocco, pp. 565– 568
Wahby I, Arráez-Román D, Segura-Carretero A, Ligero F, Caba JM, Fernández-Gutiérrez A
(2006) Analysis of choline and atropine in hairy root cultures ofCannabis sativaL. by
capillary electrophoresis-electrospray mass spectrometry. Electrophoresis 27:2208– 2215
316 I. Wahby et al.