Cannabis sativa L. - Botany and Biotechnology

(Jacob Rumans) #1

B190/B200, a codominant Sequence Characterized Amplified Region (SCAR)
marker (de Meijer et al. 2003 ), associated to and predictive of the principal
chemotypes, was developed by Bulk Segregant Analysis RAPD screening in F2s
segregating for chemotype, and was able to identify THCA and CBDA homozy-
gous and heterozygous plants belonging to these progenies (de Meijer et al. 2003 ;
Fig.15.3a, b). Nevertheless, as is common for markers developed on specific
progenies, and not directly linked to, but only associated to the gene of competence,


Fig. 15.2 The different gas-chromatographic profiles of extracts from mature inflorescences of
Cannabis sativaplants belonging to thefive different chemotypes. See text for a description of the
chemotypes


332 C. Onofri and G. Mandolino

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