Putative tetraploid plants showed significant decrease in the amount of soluble
sugars than diploid plants while the soluble sugar content in leaves of mixoploids
showed a significant increase (about twice). The changes in soluble sugars in roots
were similar to the leaves so that the amount of sugar in the roots of tetraploid
plants showed a 41% reduction, compared to the diploid plants. The highest soluble
sugar content was found in the leaves and roots of mixoploid plants (Fig.17.11).
Unlike our results, Grange et al. ( 2003 ) observed higher total sugar content in
triploid than in diploid fruit of watermelon. Increasing the amount of starch can also
be a reason to reduce the amount of sugar in the tetraploid Cannabis plant. It may be
because of the created disorders in triosephosphate translocation from chloroplast to
cytosol under these circumstances.
17.4 Conclusion
Our results showed important influence of ploidy level on all of morphological,
structural and biochemical aspects. Tetraploid plants of Cannabis was not suitable
forfiber production and medicinal usage, because tetraploid plants had lowerfiber
and cannabinoid. However mixoploid plant had intresting characterization with
higher cannabinoid, protein and sugar.
References
Allum JF, Bringloe DH, Roberts AV (2007) Chromosome doubling inRosa rugosaThunb. hybrid
by exposure of in vitro nodes to oryzalin: the effects of node length, oryzalin concentration and
exposure time. Plant Cell Rep 26:1977– 1984
Bagheri M, Mansouri H (2015) Effect of induced polyploidy on some biochemical parameters in
Cannabis sativaL. Appl Biochem Biotechnol. doi:10.1007/s12010-014-1435-8
Bhojwani SS (2004) In vitro production of triploid plants. In: Goodman RM (ed) Encyclopedia of
plant and crop science. Marcel Dekker, New York, pp 590– 593
Blanc G, Wolfe KH (2004) Widespread paleopolyploidy in model plant species inferred from age
distributions of duplicate genes. Plant Cell 16:1667– 1678
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of
protein utilizing the principle of protein-dye binding. Anal Biochem 7(72):248– 254
Buchanan BB, Gruissem W, Jones R (2000) Biochemistry and molecular biology of plants.
American Society of Plant Physiologists. ISBN 81-88237-11– 6
Chakraborti SP, Vijayan K, Roy BN, Qadri SM (1998) In vitro induction of tetraploidy in
mulberry (Morus albaL.). Plant Cell Rep 17:799– 803
Chen C, Hou X, Zhang H, Wang G, Tian L (2011) Induction of Anthurium andraeanum
‘‘Arizona’’tetraploid by colchicine in vitro. Euphytica 181:137– 145
Dhawan OP, Lavania UC (1996) Enhancing the productivity of secondary metabolites via induced
polyploidy: a review. Euphytica 87(2):81– 89
Dickison WC (1974) Trichomes. In: Radford AE, Dickison WC, Massey JR, Bell CR
(eds) Vascular plant systematics. Harper and row, New York, pp. 198–202. Oxford, pp 40– 53
17 Induction of Polyploidy and Its Effect onCannabis sativaL. 381