In general, it can be noted that pachytene chromosomes of hemp differ mor-
phologically by well distinguishable heterochromatin and using DAPI-staining in
conjunction with cytogenetic markers make it possible to develop a modern
pachytene map, which, in its turn, opens up opportunities for the physical mapping
of genes.
Fig. 18.3 Chromosomes ofC. sativawith the FISH signals (green) of CS-154 tandem repeat
(a) and of single copy fraction of scaffold 20878_8 (b)
Fig. 18.4 The meiotic pachytene chromosomes ofC. sativa.(a) The satellite chromosome 9
indicated byarrow.(b) 5S rDNA hybridization to pachytene chromosomes. The chromosome 8
with 5S rDNA FISH signal (red) indicated byarrow
392 G.I. Karlov et al.