82
Fig. 4.2 Comparison of on-target scores of 29 sgRNAs from the algorithms generated by (a)
Doench ‘16 [ 16 ], (b) Doench ‘14 [ 17 ], and (c) Moreno-Mateos [ 18 ] with their actual editing effi-
ciency in mice. A score of 30 from Moreno-Mateos’s algorithm as a cut-off (dashed line) is
recommended
C.L. Yuan and Y.-C. Hu