Quorum Sensing

2.1 Electrochemical
Workstation

1. The electrochemical cell consists of the BDD electrode
   (a diameter of 3 mm) as the working electrode, a Pt wire as a
   counter electrode, and an Ag/AgCl as a reference electrode (see
   Note 2).

Fig. 1DPV of 5μM PYO, 20μM HHQ, and 20μM PQS using 50 mM acetate

buffer (pH 5.0) consisting of 20 % ACN as the electrolyte. Detection was

performed on the BDD electrode vs. Ag/AgCl [14]

Fig. 2Monitoring the production of HHQ, PQS, and PYO in the bacterial strain

P. aeruginosaPA14 in LB media was carried out for 8 h. The bacterial culture

was extracted twice with acidified ethyl acetate and then with MCX SPE.

Electrolyte: 50 mM acetate buffer at pH 5.0 containing 20 % ACN. Detection

was performed on the BDD electrode vs. Ag/AgCl [14]

Rapid Detection ofP. aeruginosaSignal 109