- Ovine lambda chain variable region 5^0 primers.
(a) OvVL1LINKBACK: 5^0 -AG TCA AAC GCG TCT GGC
GAG TCT AAA GTG GAT GAC CAG GCT GTG CTG
ACT CAG CCG-3^0.
(b) OvVL2LINKBACK: 5^0 -AG TCA AAC GCG TCT GGC
GAG TCT AAA GTG GAT GAC CAR GCT GTG CTG
ACY CAR CYG-3^0.
(c) OvVL3LINKBACK: 5^0 -AG TCA AAC GCG TCT GGC
GAG TCT AAA GTG GAT GAC CAG GCY STG STG
ACT CAG CCR-3^0.
(d) OvVL4LINKBACK: 5^0 -AG TCA AAC GCG TCT GGC
GAG TCT AAA GTG GAT GAC MRG GTC RTG CKG
ACT CAR CCG-3^0.
(e) OvVL5LINKBACK: 5^0 -AG TCA AAC GCG TCT GGC
GAG TCT AAA GTG GAT GAC CAG KCT GYS CTG
ACT CAG CCK-3^0. - Ovine lambda chain variable region 3^0 primers.
(a) OvJL1FOR: 5^0 -ACC CAG GAC GGT CAG CCT GGT
CC-3^0.
(b) OvJL2FOR: 5^0 -ACC CAG GAC GGT CAG YCK RGW
CC-3^0. - Ovine kappa chain variable region 5^0 primers.
(a) OvVK1LINKBACK: 5^0 -AG TCA AAC GCG TCT GGC
GAG TCT AAA GTG GAT GAC GAC ATC CAG GTG
ACC CAG TCT CCA-3^0.
(b) OvVK2LINKBACK: 5^0 -AG TCA AAC GCG TCT GGC
GAG TCT AAA GTG GAT GAC GAC ATC CAG CTC
ACC CAG TCT CCA-3^0. - Ovine kappa chain variable region 3^0 primers.
(a) OvJK1FOR: 5^0 -CCG TTT GAT TTC CAC GTT GGT
CC-3^0.
(b) OvJK2FOR: 5^0 -CCT TTT GAT CTC TAG TTT GGT
TCC-3^0.
(c) OvJK3FOR: 5^0 -CCT TTT GAT CTC TAC CTT GGT
TCC-3^0. - Cloning primers (restriction sites underlined).
(a) OvVH1BACK-Sfi:5^0 -GTC CTC GCA ACT GCG GCC
CAG CCG GCC ATG GCC CAG GTK CRR CTG CAG
GRG TCG GG-3^0.
(b) OvVH2BACK-Sfi:5^0 -GTC CTC GCA ACT GCG GCC
CAG CCG GCC ATG GCC CAG GTK CAG YTK CAG
GAG TCG GG-3^0.
332 Soumya Palliyil