Quorum Sensing

3 oxo C12 BSA (50 ug/ml)

3oxo C12 BSA (100 ng/ml)

3 oxo C12 BSA (100 ng/ml)

TEA Elution (100 mM)

N-acyl C12 BSA (5 ug/ml)

N-acyl C12 BSA (100 ng/ml)

FA Elution – 1 μM 30H C12

FA Elution – 10 nM 3oxo C12

FA Elution – 1 μM 3oxo C12

3OH C12 BSA (50 μg/ml)

FA Elution – 10 μM C4-HSL FA Elution – 10 μM C4-HSL

Pan 1

Pan 2

Pan 3

Strategy 2

Strategy 1

Pan 1

Pan 2

Pan 3

Fig. 2Outline of the panning strategy adopted for the selection of cross-reactive anti-HSL clones

Monoclonal Antibodies Inactivating AHL Signal Molecules 345