Quorum Sensing

physiological pH and the antibody present in the flow-through

is collected and used for downstream purposes without any

need for neutralization. This system has been adopted because

unlike Protein A and Protein G purification systems, no elution

step is involved and thus the antibodies are not subjected to or

altered by potential harsh elution conditions.

3. Add 15% (vol/vol) glycerol and 5% (wt/vol) glucose to each
   well of the first 96-well plate (the one corresponding to the
   overnight culture) and store at 80 C as a bacterial stock.

References

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352 Soumya Palliyil