Quorum Sensing

2. Check the hydrogen bond and hydrophobic interaction pro-
   files of the selected top 10% compounds by the IDEA 8.8
   software (IDEA 8.8, 2007 Breadth Technology, Taipei, Tai-
   wan) (seeNote 3).


3. Carry out a manual binding orientation and conformation
   analysis by comparing with AI-2 to make the final 42 hits
   (Fig.4).

3.3 Quorum Sensing
Inhibition Assay

The anti-QS tests here described are performed according to previ-

ously reported procedures [32].

1.V. harveyiMM32 (ATCC# BAA-1211) is streak-seeded on a

Luria Marine (LM) plate supplemented with 50μg/mL kana-

mycin and 10μg/mL chloramphenicol, and the plate is incu-

bated overnight at 30C.

2. A single colony is picked from the LM plate and inoculated into
   2 mL of Autoinducer Bioassay (AB) medium [33]

Fig. 3A schematic illustration of the interactions of AI-2 with LuxPQ

358 Tianyu Jiang et al.