- Fuqua C, Winans SC (1996) Conserved cis-
acting promoter elements are required for
density-dependent transcription ofAgrobacter-
ium tumefaciensconjugal transfer genes. J Bac-
teriol 178:435–440 - Zhu J, Chai Y, Zhong Z, Li S, Winans SC
(2003) Agrobacterium bioassay strain for ultra-
sensitive detection of N-acylhomoserine
lactone-type quorum-sensing molecules:
detection of autoinducers in Mesorhizobium
huakuii. Appl Environ Microbiol
69:6949–6953 - Glansdorp FG, Thomas GL, Lee JK, Dutton
JM, Salmond GPC, Welch M et al (2004) Syn-
thesis and stability of small molecule probes for
Pseudomonas aeruginosaquorum sensing mod-
ulation. Org Biomol Chem 2:3329–3336 - Wood DW, Pierson LS (1996) ThephzIgene
ofPseudomonas aureofaciens30-84 is responsi-
ble for the production of a diffusible signal
required for phenazine antibiotic production.
Gene 168:49–53 - McLean RJC, Pierson LS, Fuqua C (2004) A
simple screening protocol for the identification
of quorum signal antagonists. J Microbiol
Methods 58:351–360 - Fuqua WC, Winans SC (1994) AluxR-luxI
type regulatory system activatesAgrobacterium
Ti plasmid conjugal transfer in the presence of a
plant tumor metabolite. J Bacteriol
176:2796–2806 - Zhu J, Beaber JW, More ́MI, Fuqua C, Eber-
hard A, Winans SC (1998) Analogs of the
autoinducer 3-oxooctanoyl-homoserine lac-
tone strongly inhibit activity of the TraR pro-
tein ofAgrobacterium tumefaciens. J Bacteriol
180:5398–5405 - Luo ZQ, Clemente TE, Farrand SK (2001)
Construction of a derivative ofAgrobacterium
tumefaciensC58 that does not mutate to tetra-
cycline resistance. Mol Plant Microbe Interact
14:98–103 - Zan J, Cicirelli EM, Mohamed NM, Sibhatu
H, Kroll S, Choi O et al (2012) A complex
LuxR-LuxI type quorum sensing network in a
roseobacterial marine sponge symbiont acti-
vates flagellar motility and inhibits biofilm for-
mation. Mol Microbiol 85:916–933 - Shaw PD, Ping G, Daly SL, Cha C, Cronan JE
Jr, Rinehart KL et al (1997) Detecting and
characterizingN-acyl-homoserine lactone sig-
nal molecules by thin layer chromatography.
Proc Natl Acad Sci U S A 94:6036–6041 - Stauff DL, Bassler BL (2011) Quorum sensing
inChromobacterium violaceum: DNA recogni-
tion and gene regulation by the CviR receptor.
J Bacteriol 193:3871–3878
40. Blosser RS, Gray KM (2000) Extraction of
violacein from Chromobacterium violaceum
provides a new quantitative bioassay forN-
acyl homoserine lactone autoinducers. J Micro-
biol Methods 40:47–55
41. Stoodley P, Sauer K, Davies DG, Costerton JW
(2002) Biofilms as complex differentiated com-
munities. Annu Rev Microbiol 56:187–209
42. Donlan RM, Costerton JW (2002) Biofilms:
survival mechanisms of clinically relevant
microorganisms. Clin Microbiol Rev
15:167–193
43. Petrova OE, Sauer K (2016) Escaping the bio-
film in more than one way: desorption, detach-
ment or dispersion. Curr Opin Microbiol
30:67–78
44. Yang L, Givskov M (2015) Chemical biology
strategies for biofilm control. Microbiol Spectr
3:MB-0019-2015
45. Kim SK, Lee JH (2016) Biofilm dispersion in
Pseudomonas aeruginosa. J Microbiol
54:71–85
46. Davies DG, Marques CNH (2009) A fatty acid
messenger is responsible for inducing disper-
sion in microbial biofilms. J Bacteriol
191:1393–1403
47. Hengge R (2009) Principles of c-di-GMP sig-
nalling in bacteria. Nat Rev Microbiol
7:263–273
48. Li Y, Petrova OE, Su S, Lau GW, Panmanee W,
Na R et al (2014) BdlA, DipA and induced
dispersion contribute to acute virulence and
chronic persistence ofPseudomonas aeruginosa.
PLoS Pathog 10:e1004168
49. Barraud N, Schleheck D, Klebensberger J,
Webb JS, Hassett DJ, Rice SA et al (2009)
Nitric oxide signaling inPseudomonas aerugi-
nosa biofilms mediates phosphodiesterase
activity, decreased cyclic diguanosine-5^0 -mono-
phosphate levels and enhanced dispersal. J Bac-
teriol 191:7333–7342
50. Rice SA, Tan CH, Mikkelsen PJ, Kung V, Woo
J, Tay M et al (2009) The biofilm life cycle and
virulence of Pseudomonas aeruginosa are
dependent on a filamentous prophage. ISME
J 3:271–282
51. Fuqua C, Burbea M, Winans SC (1995) Activ-
ity of theAgrobacteriumTi plasmid conjugal
transfer regulator TraR is inhibited by the
product of the traM gene. J Bacteriol
177:1367–1373
52. Tempe J, Petit A, Holsters M, Von Montagu
M, Schell J (1977) Thermosensitive step asso-
ciated with transfer of the Ti plasmid during
conjugation: possible relation to transforma-
tion in crown gall. Proc Natl Acad Sci U S A
74:2848–2849
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