RNA Detection

design offered cooperative hybridization between the capture

probes on the solid surface and the multiple CEs binding to

one target, presumably leading to a stronger capture than

single CE–capture probe interaction [5], which makes sure

that only the specific target can be captured to the solid surface

and further trigger downstream cascade reactions.

13. As the hybridization is taken place at a temperature (58C)
    that is higher than the annealing temperature of single CE or
    LE probe, single probes or hairpins will be washed off and
    cannot be stably immobilized on the solid surface to cause
    nonspecific absorption. Leaky hairpin polymerization products
    without binding to the target will be washed off as well without
    causing background.

References

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3. Dong J, Cui X, Deng Y, Tang Z (2012) Ampli-
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196 Yao Xu and Zhi Zheng