RNA Detection

4. Microinjection buffer: 48 mM K 2 HPO 4 , 4.5 mM KH 2 PO 4 ,
   and 14 mM NaH 2 PO 4 , pH 7.2 (seeNote 2).


5. Glass bottom dish (e.g., MatTek).


6. Microcentrifuge.

2.5 Microscope and
Imaging Software

1. An inverted widefield fluorescence microscope. We use an
   Olympus IX83 Motorized inverted fluorescence microscope
   equipped with a back-illuminated EMCCD camera (Andor)
   and an MT-20E excitation source (Olympus).


2. 400.95NA objective lens.


3. Filter set for DAPI, EGFP, and TAMRA (e.g., Olympus
   MT20).


4. Filter set for Cy5 (ET620/60, ET700/75m, and T660lpxr).


5. Filter set for IRDye®800 (ET710/75x, ET810/90m, and
   T760lpxr).


6. cellSens Dimension image acquisition software (Olympus).


7. Fiji software for image analysis (seeNote 3).

2.6 Synthesis of
Reference-Dye
Labeled Dextran

1. IRDye®800-NHS ester (e.g., Li-Cor) dissolved in anhydrous
   DMSO or DMF (seeNote 4).


2. Reaction buffer: 50 mM sodium borate buffer, pH 8.


3. Elution buffer: 48 mM K 2 HPO 4 , 4.5 mM KH 2 PO 4 , and
   14 mM NaH 2 PO 4 , pH 7.2.


4. Aminodextran solution: 2.5 mg/mL aminodextran
   (MW¼10 kDa) in reaction buffer.


5. Gel chromatography columns (e.g., GE Healthcare NAP-5).


6. Centrifugal filter units, YM-3, 3000 MW cutoff (e.g., Millipore
   Centricon).


7. Microcentrifuge.


8. Thermomixer.


9. Spectrophotometer (e.g., Thermo Scientific BioMate 3S).

2.7 Preparation of
Microemulsion
Samples

1. Span-80.


2. Tween 80.


3. Mineral oil.


4. 5 mL glass vials.


5. 50 mL tubes.


6. Magnetic stirrer and magnetic stir bars.


7. Glass pipettes.


8. Electronic scale.

246 Mingming Chen et al.