Fig. 2In vivo electroporation procedure to deliver ECHO probes into living mouse brains. (A) Our experimental
setup to conduct in vivo electroporation in P7–9 mice. (B) An anesthetized mouse stabilized on a plastic box lid
with injecting/anode needle in its cerebellum and a pair of cathodes pressed against its ears. (C) Illustration of
injection site and current delivery procedure.Green dots: probe injection sites (0.5 mm from the surface). (D)
Dorsal fluorescence view of P7 cerebellum electroporated with D514-d(T) 12. (E) An open-book configuration of
electroporated brain on a glass-bottom dish to reveal the inner layers of cells. Fast green dye can be used to
266 Dan Ohtan Wang