Fig. 3Target recognition specificity validated by fluorescence patterns and colocalization with protein
markers. (A) Preparation illustration of acute brain slices from electroporated mice. (B) A representative
confocal image from acute cerebellar slices prepared soon afterin vivoelectroporation. EGL: external granule
layer, ML: molecular layer, PL: Purkinje layer, IGL: inner granule layer. (C) Confocal images of poly(A), U3
snoRNA, and 28S rRNA in individual cerebellar granule cells after electroporation. Intranuclear foci containing
target RNA concentrations are readily distinguished. The number and shape are consistent with foci of nuclear
speckles and nucleoli. (D) Electroporation of oligonucleotide probes labeled with a conventional dye Cy5-d(T) 30
268 Dan Ohtan Wang