- Thermocycler.
- qPCR machine.
- 1.5/2 mL tube thermomixer.
- 1.5 mL centrifuge.
- Vacuum pump.
- Sonicator.
- Magnetic rack.
- Acetate printing film.
- Printer.
- Near infrared imager (seeNote 1).
- Cell scrapers.
- 15 mL centrifuge tubes.
- 2 mL microcentrifuge tubes.
- 1.5 mL microcentrifuge tubes.
- Low-binding 0.5 mL microcentrifuge tubes (e.g., Thermo-
Fisher AM12350). - Low-binding 1.5 mL microcentrifuge tubes (e.g., Thermo-
Fisher AM12450). - PCR tubes.
- qPCR tubes/microplates.
- Sterile filters.
- Protran 0.45 nitrocellulose membrane.
- Whatman filter paper.
- Razor blades.
- 30 G syringe needles.
- 16 G syringe needles.
- Phase-lock heavy columns.
- Costar-X filter spin columns (e.g., VWR International).
- Proteus Clarification columns (e.g., Generon).
- Glass prefilters.
2.2 Required
Reagents and
Solutions
- 1PBS.
- Tris–HCl, pH 7.4.
- Tris–HCl, pH 6.5.
- Tris–HCl, pH 7.8.
- NaCl solution.
- Igepal CA-630.
- SDS.
iCLIP to Determine Protein-RNA Interactions 433