Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

Table 8.1

Examples of endophytic actinobacteria isolated from different crops and respective isolation strategy

Crop

Planttissue

Sterilization methodemployed

Strategy of planttissue inoculation

Isolation medium

Antimicrobialsupplementedto growthmedium

Number ofrecoveredendophyticactinobacterialisolates

Identi

fication of

endophyticactinobacterial isolates

References

Maize (

Zea mays

L.)

Leavesand roots

Ethanol 70% for30 sSodiumhypochlorite (3

• 
  

5%) for 3 minFinal rinse withsterile water

Roots cut into ca.1 cm fragments andleaves divided in ca.1cm

2

fragments

Starch-casein-agar2.5% water-agar

Nystatin andcycloheximide(50

μg/ml)

53

Microbisporaspp.

Streptomyces

spp.

Streptosporangium

spp.

Ara

újo et al.

(^2000

)

Wheat (

Triticum

aestivum

L.)

Roots

Ethanol 99% for60 sSodiumhypochlorite(3.125%) for 6 minFinal rinse in sterilereverseosmosis-treatedwater

Cut into 1 cmfragments

Tap water

• yeast

extract agarHumic acid

• vitamin B

agarFlour

• yeast
  extract-sucrose-caseinhydrolysate agarFlour-calciumcarbonate agarYeast extract-caseinhydrolysate agar

Benomyl

58

Streptomyces

spp.

Micromonospora

spp.

Nocardiodes albusStreptosporangium

/

Microbispora

Coombs andFranco (

2003

)

Tomato(Lycopersiconesculentum

)

Roots

Ethanol 70% for5 minSodiumhypochlorite (2.5%)for 10 minFinal rinse withsterilized distilledwater

Cut into 1 cmfragments

Starch casein agarS mediumISP2 agar

None

70

Streptomyces

spp.

Micromonospora

spp.

Microbispora

spp.

Nocardia

spp.

de Oliveiraet al. (

2010

)

(continued)

168 M.F. Carvalho et al.