Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

(Tina Meador) #1

compounds/metabolites has several advantages. For example, (i) When a plant


based drug is produced from the source like endophytic fungi, it will not only


reduce our dependence on plant sources for these metabolites but also help in


preserving the continuously decreasing plant biodiversity on earth (Strobel et al.


2004 ). (ii) Environmental conditions which affect the quality and quantity of


desired phytochemicalsin plantacan be avoided if isolated endophytes are cultured


successfully in vitro by providing optimum physicochemical parameters.


(iii) Production of plant derived drugs using microbial sources can be easily scaled


up for industrial process. It is also possible to obtain improved versions of existing


drugs by simply altering the cultural conditions. (iv) Isolation of drugs from


microbial sources is relatively easier and cost-effective as compared to extraction


and purification of drugs from the whole plant or plant part(s) including tissue


culture. (v) Since the plant and fungal endophytes, both are eukaryotic systems, the


metabolites produced by the endophytes are less likely to show mammalian toxicity


when used for human applications and, (vi) High-value metabolites/compounds of


medicinal importance are produced in easier and economical way using the


endophytic microorganisms (Strobel et al. 2004 ).


It has been proved that both plant and endophytic fungi isolated from the same


plant produced similar compounds with same bioactivity (Kusari et al. 2012 ). The


possible reason for this could be the genetic recombination between the host and
endophytes or vice versa that occurred during the course of evolution (Tan and Zou


2001 ).


In the ensuing paragraphs, an attempt has been made to briefly review the studies


on plants and endophytic fungi which could produce antioxidant, antihyperc-


holesterolemic, antidiabetic, and anticancer metabolites of therapeutic importance.


10.2 Antioxidant Activity of Plants/Plant Extracts


Reactive oxygen species (ROS) generated in the biological system are the major cause


of the degenerative conditions such as aging, cancer inflammation, atherosclerosis, etc.


(Sandesh et al. 2014 ). Naturally occurring antioxidants/enzymes, superoxide dismu-


tase, catalase, glutathione, etc., can stabilize the adverse effects of ROS and, thus help


maintain the redox balance in the body. However, when generation of ROS is more


than what can be processed by the endogenous antioxidant system, it results in


oxidative damages leading to several other disorders such as arthritis, atherosclerosis,


cancer, diabetes, and many others (Castaneda et al. 2003 ). Sufficient amounts of


exogenous antioxidants are required to reduce the effects of ROS to the human body. In


response to the growing consumer demand for food supplements that are free of


synthetic antioxidants with carcinogenic potential (Baardseth 1989 ), there has been


tremendous increase in the search for naturally occurring antioxidants during the past


decades (Gould 1995 ). Of the vast amount of literature, a concise list of studies on the


antioxidant activities of plant/plant parts using DPPH free radical scavenging assay,
mainly in last 10 years is shown in Table10.1.


10 Endophytes: Potential Source of Therapeutically... 215

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