Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

(Tina Meador) #1

12.4.2 Effect of Fungal Pre-treatments on Kraft Pulping


Fungal pre-treated chips with endophytes and saprophytic fungi were subjected to


kraft pulping (Fig.12.5). Lower Kappa number and higher brightness values were


obtained for all the samples treated with fungi compared to control pulp (without


fungal treatment). However, no significant differences in polymerization degree


were found. Moreover, higher delignification was observed with endophytic strains
during kraft pulping compared toTrametessp. I-62, exceptP. smilacis. The highest


increment in delignification (27% compared to control) was found when


Hormonemasp. was applied compared with 9% obtained byTrametessp. I-62.


Moreover,Hormonemasp. andN. australeprovided higher brightness (46.0 and


44.3% ISO, respectively) thanTrametessp. I-62 (41.6% ISO). Other white-rot


fungi have improved delignification during kraft pulping in previous works, but the


results were worse than that observed for this endophytic fungus in most of the


cases: when chips from hardwood (eucalyptus and poplar) were pre-treated with


Ceriporiopsis subvermisporaan increase of 14% was obtained (Yadav et al. 2010 );


increases around 18% were produced from pine pre-treated withPycnoporus


sanguineus,Pycnoporussp. andStereumhirsutum(Wolfaardt et al. 2004 ). Higher


increases were measured when soda pulping was performed in rice straw pre-treated


withP. chrysosporiumandPleorotus ostreatus: 26 and 35%, respectively (El-Din


et al. 2013 ).


Among all the strains studied,Hormonemasp. showed the minor NaOH con-


sumption during pulping, 12% less than control and similar Na 2 S consumption.


N. australepre-treated samples consumed also around 8% less NaOH than control


andTrametessp. I-62 samples. However, Na 2 S consumption increased from 54 to


82%, and kappa number and brightness were worse than those found for pulps


pre-treated withHormonemasp. These reductions in the alkali dose necessary to


produce a target kappa number in chemical pulps have been associated with the


modifications in wood chips caused by fungi (Mardones et al. 2006 ).
Mechanical properties of handsheets of the obtained pulps were improved when


a fungal pre-treatment was performed before pulping. Endophytic fungi, except


Fig. 12.5 Scheme of the experimental procedure of biological pre-treatments to enhance Kraft
and mechanical pulping


274 Ú. Fillat et al.


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