Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

(Tina Meador) #1

protoplasm fusion of endophyteMucorsp. in rape roots contaminated the soil with


Pb and Cd.


Although heavy metals are toxic to plants, it has been demonstrated that many


plants are metal tolerant and some of them are metal hyper accumulators (Rosa


et al. 2004 ; Li et al. 2012 ). Many metal resistant endophytes were isolated from


hyperaccumulating plants. These fungi belonged to various taxa include


Microsphaeropsis,Mucor, Phoma, Alternaria, Pyronellaea, Steganosporiumand


Aspergillus. Soleimani et al. ( 2010 ) demonstrated that endophytic fungi were


helpful in phytoremediation of aged petroleum contaminated soil and that these


fungi improved host plants roots and shoot biomass and created higher levels of


water soluble phenols and dehydrogenase activity in the soilTCE, Naphthalene,


BTEX, catechol and phenol could be degraded by endophytes which decreased the


contaminant phytotoxicity and improved plant growth (Weyens et al. 2010 ;Ho


et al. 2009; Li et al. 2012 ).


3.5 Endophytic Fungi in Bio Degradation of Pollutants


Fungi are known to utilize a wide range of organic compounds for nutrition and
energy generation through extracellular enzymes. These organic compounds


include cellulose, pectin, lignin, lignocelluloses, chitin and starch and anthro-


pogenic substances such as hydrocarbons, pesticides and other xenobiotics. The


white rot fungi likePhaenerochate chrysosporiumcan degrade several xenobiotics


such as aromatic hydrocarbons, chlorinated organics, poly chlorinated biphenyls,


nitrogen containing aromatics and many other pesticides, dyes and xenobiotics


(Gadd 2007 ; Harvey and Thursten 2009 ).


The use of fungi to clean up environmental pollutants has gained the momentum


in past few years, however most studies have focussed on white rot fungi


(Marco-Urrea et al. 2008 ; Nikiforova et al. 2009 ) and the use of endophytic fungi


might be a novel approach and important source for degradation of toxic pollutants


which includes hydrocarbons, polychlorinated biphenyls (PCBs), polyaromatic


hydrocarbons (PAHs), radionuclides and metals.


Fungi are known to degrade PAHs in surface of soil. These fungi produce


extracellular enzymes with lower substrate specify which enable degrade aromatic


compounds including PAHs (Leonardi et al. 2007 ; Farnet et al. 2009 ). In a study,


manganese peroxidase was found to be the dominant ligninolytic enzyme in the


degradation PAH (Tian et al. 2007 ; Dai et al. 2007 ). Researchers found that


endophytic fungi might be a novel and important resource for the degradation of


polycyclic aromatic hydrocarbons (PAHs). An endophyte fungal strain


Ceratobasidium stevensiiisolated from the plant ofEuphorbiaceaewas found to


metabolize phenanthrene effectively (Dai et al. 2010 ). Tian et al. ( 2007 ) demon-


strated the degradation of phenanthrene by endophyticPhomopsissp., with rice


plant. In a study, endophyticXylariaceaestrains isolated from healthy tropical
native plants of Thailand found to be the producers of ligninolytic enzymes


54 Y.L. Krishnamurthy and B.S. Naik

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