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Stepwise addition of components that recapitulate human cerebellar development
induced the generation of continuous cerebellar-like NE that are stratified as seen in
the early cerebellar plate. Here I discuss the self-organizing nature of hPSC-derived
cerebellar tissues with regard to spontaneous polarity formation in 3D culture. I also
discuss applications to intractable cerebellar disease utilizing patient-specific iPSCs.
Fig. 2.2 Schematic diagram showing early patterning of the neural tube. (a) Lateral view of
embryonic brain showing the distribution of secreting signaling molecules. Te l telencephalon, di
diencephalon, mes mesencephalon, sl sulcus limitans, is isthmus, cp choroid plexus, fp floor plate,
bp basal plate, ap alar plate, and r1-8 rhombomere 1-8. (b) Simplified diagram showing the axial
structure of the neural tube. The neural tube, which initially has an anterior identity, is subse-
quently patterned into discrete domains by secreted factors along the anteroposterior and the dor-
soventral axes. ANR anterior neural ridge, DA dopaminergic neurons, and 5HT serotonergic
neurons
K. Muguruma