Organ Regeneration Based on Developmental Biology

86

A)
Bioengineered tooth eruption
Day 16

Day 37

Day 49

Bioengineered tooth

occlusion

B)

NT BT

PDL

NT AB BT

PDL

Occlusion of a bioengineered tooth unit HE analysis of a bioengineered tooth unit Higher magnification

HE analysis of a erupted bioengineered tooth

Day (^16) Day 31 Day 83
Scale bar 200 μm Scale bar 200 μm
Scale bar 100 μm
Scale bar 200 μm
Scale ba r 500 μm
Fig. 5.5 Whole-tooth regeneration through the transplantation of a bioengineered tooth germ or a
bioengineered tooth unit. (a) The transplanted bioengineered tooth germ erupted (arrowheads,
upper-left panels) and reached the occlusal plane with the opposing lower first molar at 49 days
after transplantation (upper-right panels). The bioengineered tooth also formed a correct tooth
structure comprising enamel, dentin, dental pulp and periodontal tissue (lower panels). (b) The
bioengineered tooth unit was engrafted through bone integration and reached the occlusal plane
with the opposing upper first molar at 40 days after transplantation (arrowheads, left panels). The
engrafted bioengineered tooth unit also had the correct tooth structure in the recipient jaw. NT,
natural tooth; BT, bioengineered tooth; AB, alveolar bone; PDL, periodontal ligament (centre and
right panels)
M. Oshima and T. Tsuji