Primary Osteintegration in the Study of Biomimetic Surfaces
99
Fig. 1. Histological results of HVP (351-359) trials. (a-b) Goldner’s trichrome stain (original
magnification, 40x). Primary new bone directly apposed to the interface. (a) The picture
corresponds to an HVP samples belonging to the 9D group; (b) the picture corresponds to
an HVP samples belonging to the 16D group. Arrows show newly formed bone
characterized by round shape osteocytes. (c) Polarized light microscopy with compensation
plate (original magnification, 40x). Woven fiber bone located at the interface in the HVP
sample, belonging to 16D group. Arrows indicate the woven fiber bone. (d) Fluorescent
microscopy of CG and CB fluorochromic bone marker (original magnification, 20x). The
picture represents the direct bone apposition to the interface the days immediately after
surgery (Calcein green bone marker) and the following bone apposition, marked with
Calcein Blue in the HVP sample belonging to 16D groups.