Biomimetic Structured Porogen Freeform Fabrication System for Tissue Engineering
73
(A) (B)
Fig. 17. Designed fixture for tensile testing: (A) Two ends of the scaffold holder (B) Top view
of single scaffold holder to show the cavity for mounting the scaffold.
(A) (B)
Fig. 18. (A) PCL scaffold for tensile testing (B) CAD design of porogen to make long scaffold
for testing.
2.5 Biocompatibility test
To test the cytocompatibility of the scaffolds made by our structured porogen method using
DDP system human embryonic palatal mesenchymal (HEPM) cells (ATCC, CRL-1486) were
used in this part of study.
2.5.1 Testing protocols
HEPM cells are routinely maintained in Eagles’ minimum essential medium (MEM) with
Earles’ salts supplemented with 10% fetal bovine serum (Hyclone), 2.0mM L-glutamine,
1.0mM sodium pyruvate, 0.1 mM non-essential amino acids, and 1.5 g/L sodium
bicarbonate at 37°C in a 5% CO 2 incubator [Li et al. 2005]. For cell culture studies, the