Caspases,Paracaspases, and Metacaspases Methods and Protocols

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Ab: h14D146

Procaspase-14 concentration (μg/ml)

0

0.05

0.1

0.15

0.2

0.25

0.05 0.1 0.15

Absorbance at 405 nm

Ab: h14D146

R^2 = 0.993

0

0.2

0.4

0.6

0.8

1

1.2

1.4

0.05 0.1 0.15 0.2

Absorbance at 405 nm

revC14 concentration (μg/ml)

R^2 = 0.974

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

a

b

c

0.5 1.0 1.5

Procaspase-14 concentration (μg/ml)

Ab: H99

Absorbance at 405 nm

Fig. 2 Two types of caspase-14 ELISA assays. ( a ) Standard curve obtained from

total caspase-14 measurement using H99 antibody. ( b ) Standard curve obtained

from active caspase-14 (mature form) measurement using h14D146 antibody.

( c ) Proof of specifi city for ELISA assay B showing that cleavage-site-directed

antibody, h14D146, does not react with procaspase-14

Mami Yamamoto-Tanaka and Toshihiko Hibino

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