Caspases,Paracaspases, and Metacaspases Methods and Protocols

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9. EGTA is added to the Laemmli buffer to sequester the Ca 2+
   and improve the SDS-PAGE.


10. Don’t cover the gel with plastic foil as this will obstruct the
    signal and keep the gel as straight and close to the screen as
    possible in order to enhance the sharpness of the image (for
    example by the addition of additional papers behind the gel).

Acknowledgements

S.S. is indebted to the Special Research Fund of Ghent University

for a postdoctoral fellowship. F.V.B acknowledges support from

grants of the Ghent University Multidisciplinary Research

Partnership “Ghent BioEconomy” 27 (project 01MRB510W), the

Belgian Science Policy Offi ce (project IAP7/29), and the Research

Foundation Flanders (FWO-Vlaanderen; project G.0038.09).

E.A.M. and P.V.B. acknowledge support from grants of the

Swedish Research Council (VR), Knut and Alice Wallenberg

Foundation, the Swedish Foundation for Strategic Research (SSF),

Pehrssons Fund and Olle Engkvist Byggmästare Foundation.

References

Elena A. Minina et al.

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