CED-9 ............................................................. 102, 105, 106
Cell free system ........................................................ 1 19, 120
Cell lysate ...................................................2 2, 23, 70, 73, 74,
84, 172, 190, 191, 194, 198, 219, 238, 241, 243,
246–247
Chemically induced dimerization (CID) domains .............3 6
Chromophore .................. 1 5, 21, 42, 122, 129, 145, 146, 159
Clan CD peptidases ................................................. 2 04, 205
Cleavage-site directed antibody .............................. 9 0, 94–98
Cleavage-site mutant .............................................. 2 6, 27, 36
Cleaved-Caspase-3 ................................................... 111–113
Combined fractional diagonal chromatography
(COFRADIC) ................................ 2 49, 255–260
Corneocyte ................................................. 9 0, 91, 93–95, 99
Cryoprotection ......................................... 2 07–208, 212–213
Custom Perl scripts ............................................................6 4
Cyan fluorescent proteins (CFP) ................................ 2 9, 186
Cysteine peptidase .................................................... 2 03–205
Cytochrome c ............................................119, 120, 123, 124,
126, 130–133, 136, 158
Cytokeratin-18 (CK18) ................................... 1 42, 144, 151,
158–159, 162, 163, 167, 168
D
DAPI ............................. 9 2, 97, 111, 115, 144, 150, 162, 167
Dcp-1 ....................................................................... 109, 110
Death effector domain (DED) ............................. 1 9, 26, 156
Death-inducing signaling complex
(DISC) ............................................... 1 9, 91, 146
Degradomics ................................................................ 3 , 257
Densitometry ....................................................................2 30
Deparaffinization ................................................ 9 7, 149, 152
3,3′-Diaminobenzidine (DAB) .......................... 9 9, 144, 148
Dimerisation .......................................................... 1 9, 20, 27,
36, 71, 72, 74, 157, 180, 185, 204, 238
DNA fragmentation ................................................. 1 25, 158
DNA staining ........................................... 2 25, 226, 232–233
DrICE ...................................................................... 1 09, 110
Dronc ............................................................... 1 09, 110, 113
DTASelect software ............................................... 6 4, 66, 67
E
Edelhoch relationship ................................................... 1 0, 31
Egg extract ............................................................... 1 19–139
ELAV ....................................................................... 1 14, 115
ELISA ...................................................... 9 0, 91, 96–98, 151
End point kinetics ..............................................................3 2
Epidermis ...........................................................................8 9
Exosite ................................................................................3 7
F
Fast protein liquid chromatography (FPLC) ......................3 0
Filter trap assay ......................................... 2 25–226, 228–230
Fixation ....................................... 9 7, 103, 110, 114, 152, 160
FLAG epitope ..................................................................1 90
Fluorescence activated cell sorting (FACS) .............. 1 63, 185
Fluorescence resonance energy transfer
(FRET) ................................... 1 80, 182, 184–185
Fluorochrome-labeled inhibitors of caspases
(FLICA) ................................................. 1 42, 160
Fluorogenic library .............................................................4 4
Fluorophore .................................... 1 5–17, 31, 41, 42, 44, 54,
56, 111, 112, 115, 148, 153, 214
Fmoc-amino acid ...............................................................4 3
Fractionation .............................................. 6 2, 122, 126–127
G
Gel shift assay ........................................................... 2 09, 218
GraphPad Prism software ........................................... 5 6, 208
Green fluorescent protein (GFP) ............................. 1 14, 186
H
Heat stress ................................................ 2 24, 226–227, 233
Heavy atom soaks ..................................... 2 07–208, 213–214
High performance liquid chromatography
(HPLC) .............................4 6, 54, 63, 65, 66, 257
His-tag fusion protein .......................................................... 7
Homogenization ............................................... 1 42–147, 151I
IAP2-MALT1 fusion ............................................... 1 77–179
ICAD/DFF45 ..................................................................158
IKK complex ....................................................................1 78
IMAC purification ................................................. 4 , 11, 210
Imaginal disc ............................................................ 1 09–115
Immobilized metal affinity chromatography
(IMAC) ................. 4 , 9, 11, 30, 37, 207, 210, 219
Immunofluorescence ........................................ 1 48, 150, 153
Immunohistochemistry .............................................. 9 1, 151
Immunolabeling ....................................................... 1 10–112
Immunoprecipitation .........................1 71, 224, 226, 230–231
Inflammasome ....................................................................9 1
In-gel digestion ...................................................... 6 3–66, 70
Inhibitors of apoptosis proteins (IAPs) .................... 1 51, 159
In vitro translation .............................................. 7 7, 105, 106
Ischemia ................................................................... 1 45, 147K
Kallikrein-related peptidase ................................................9 0
Keratin .................................................................... 7 0, 89, 90
Keratinocyte ................................................................. 8 9, 90
Kosmotropic salt ................................ 2 0, 36, 45, 99, 180, 186L
Lamin A ................................................... 1 42, 144, 151, 162
Leupeptin .......................................................... 2 8, 121, 126,
127, 137, 142, 143, 191, 192, 194, 198, 240264
CASPASES, PARACASPASES, AND METACASPASES: METHODS AND PROTOCOLS
Index
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