Drug Metabolism in Drug Design and Development Basic Concepts and Practice

13.Amino

acidconjugation

OH

O

NH

O

OH

O

Glycine

Hippuric Acid

Benzoic Acid

The proton signal for the

methylene proton wasat

d4.3 ppm, compared

with the same protonfor glycine at

d3.8 ppm

No further experiments are

needed

The amide proton (

d9.4, t,

1H) was also observed

All the aromatic protons

were present

Integration of all the

peaks was consistent

403