Front Matter

130 8 Production of Functional Lipids Containing Polyunsaturated Fatty Acids with Lipase

Figure 1. Time course of selective hydrolysis of tuna oil withCandida rugosalipase. (A) Extent of
hydrolysis. (B) Main fatty acid contents in undigested glyceride fraction. The fatty acid contents in gly-
cerides were expressed relative to those in the original tuna oil.&, Palmitic acid (the initial; content,
18.2 %); ^, palmitoleic acid (4.6 %); ^, stearic acid (4.4 %); &, oleic acid (18.5 %); ,
EPA (6.9 %);, DHA (24.4 %).

Figure 2. Correlation between hydrolysis extent of tuna oil and DHA content in undigested glycerides.
Tuna oil was hydrolyzed at 35 8 C for 24 h in a mixture containing 50 % water and with 20 to 2000 U g–1
mixture ofCandida rugosalipase.