138 8 Production of Functional Lipids Containing Polyunsaturated Fatty Acids with Lipase
Figure 5. Triglyceride components of original tuna oil (A) and transesterified oil (B). HPLC analysis
was performed on two ODS columns (4.6150 mm). The sample was eluted with a mixture of acetone/
acetonitrile (1 : 1, v/v) at 40 8 C and a flow rate of 0.4 mL min–1. Tricaprylin was eluted at 11.8 min under
these conditions. 8, CA; D, DHA; E, EPA, O, oleic acid; P, palmitic acid.
Table 5.Continual batch acidolysis of tuna oil with caprylic acid (CA) usingRhizopuslipase.
Fatty acid composition in glycerides (mol%)Cycle number
8 : 0 16 : 0 18 : 1 20 : 5 22 : 6
1 42.5 10.8 5.3 3.4 22.8
5 42.9 10.4 5.1 3.8 22.710 42.9 10.6 5.3 3.9 21.9
15 40.5 11.3 5.6 3.4 22.9
20 30.7 14.9 8.7 4.8 22.6
21 42.4 10.6 5.3 3.8 22.2
Reactions except for the 21st cycle were performed for 2 days, and the 21st reaction was done for 4 days.