content incorporated into triglycerides did not change for 60 days, and then gradually
decreased. The CA content in glycerides was decreased to 40 mol% after 100 days,
but returned to the initial level after the flow rate was reduced to 3.1 mL h–1. This
result showed that the immobilized lipase could be used for at least 100 days. The
daily amount of substrate converted to structured lipid by 1 g of the lipase was 13.5
mL g–1per day. To compare the efficiency in the flow reaction with that in the batch
140 8 Production of Functional Lipids Containing Polyunsaturated Fatty Acids with Lipase
Figure 6. Fixed-bed bioreactor for production of GLA-containing MLM-type structured lipid. 1, Stor-
age vessel of substrate (300 mL); 2, glass column packed with 8 g immobilizedRhizopus delemarlipase
(1562 mm); 3 peristaltic pump; 4, receiver of eluted reaction mixture (500 mL).
Figure 7. Stability of immobilizedRhizopuslipase in a continuous flow and a continual batch reacti-
ons., Continuous flow reaction. A substrate mixture (borage oil/CA¼1 : 2, w/w) was fed into a column
packed with 8 g immobilized lipase (1562 mm) at 30 8 C and 4.5 mL h–1flow rate. After 100 days, the
flow rate was reduced to 3.1 mL h–1., Continual batch reaction. A mixture of 13.5 mL borage oil/CA
(1 : 2, w/w) and 0.5 g immobilized lipase was incubated at 30 8 C for 2 days with shaking. The reaction was
repeated by transferring the lipase into a fresh substrate mixture. After 100 days, the reaction period was
extended to 3 days.