taining PUFA onto a capillary column in order to avoid sample deterioration before
entering the column. Careful programming of the column temperature is required to
achieve a good peak separation. For quantitative analysis,n-eicosane can be used as
an internal standard. Figure 3 shows a GC chromatogram of the reaction mixture
shown in Equation (1) using a non-regiospecific lipase (NovozymeTM) (Han et
al., 1999a). Changes in the content of TAG-A during the time course of the reaction
can be conveniently monitored by GC analysis.
In HTGC analysis of a TAG fraction obtained from the reaction shown in Equation
(3), TAG species are separated depending on their carbon numbers; the composition
158 9 Lipase-Catalyzed Synthesis of Structured Triacylglycerols
Figure 4. Gas chromatograms of the TAG fraction [see Equation (3)] (Iwasaki et al., 1999). (A) From
single cell oil (SCO). (B) From the reaction byRhizomucor mieheilipase. (C) From the reaction by
Pseudomonaslipase. The numbers of carbon atoms for the major peaks are indicated after the symbol
C. For (B) and (C), the contents of TAG species (% peak area) are shown in parentheses.